Fig. 1: OTUD7B depletion decreases ERα signaling activity in breast cancer cells.
From: OTUD7B stabilizes estrogen receptor α and promotes breast cancer cell proliferation
A The siRNAs specific to each deubiquitinating enzyme were transfected into MCF-7 cells. After 48 h, cells were lysed and the ERα protein level was analyzed by Western blot. Relative ERα protein level was normalized to GAPDH. B OTUD7B depletion decreased ERα protein level. C OTUD7B depletion did not affect ERα–mRNA level. D OTUD7B WT or C194S was transfected into MCF-7 cells and ERα expression was detected. E, F OTUD7B depletion decreased ERα target genes in the absence or presence of estrogen. Breast cancer cells were transfected with siOTUD7B or siControl. After 48 h, cells were treated with either ethanol or 10 nM estrogen for 6 h. Total RNA was prepared, and the expression of the endogenous ERα target genes, PS2, GREB1, and PDZK1, was determined by qRT-PCR. G, H OTUD7B depletion affected ERE-luciferase activity. Breast cancer cells were transfected with siOTUD7B or siControl together with ERE-luciferase reporter plasmid. Cells were treated with 10 nM estrogen or vehicle. Luciferase activity was measured 48 h after transfection. *P < 0.05, **P < 0.01, ***P < 0.001.
