Fig. 5: OTUD7B increases ERα stability.
From: OTUD7B stabilizes estrogen receptor α and promotes breast cancer cell proliferation
A In the presence of the proteasome inhibitor MG132, depletion of OTUD7B did not further decrease the ERα protein level. Breast cancer cells were transfected with siOTUD7B or siControl. After 48 h, cells were treated with 10 µM MG132/vehicle for 6 h; cell lysates were prepared for western blot analysis. B MCF-7 cells were transfected with OTUD7B (wild type or C194S) together with OTUD7B siRNA. The ERα levels were measured. C OTUD7B depletion decreased ERα half-life in breast cancer cells. Breast cancer cells were transfected with siOTUD7B or siControl. After 48 h, cells were treated with 100 µM cycloheximide/vehicle for indicated times. Cell lysates were prepared for western blot analysis. D OTUD7BC194S did not increase ERα half-life in HEK293 cells. HEK293 cells were transfected with HA-ERα plasmid and Myc-tag, Myc-OTUD7B, or Myc-OTUD7BC194S plasmids. After 24 h, cells were treated with 100-µM cycloheximide/vehicle for indicated times. Cell lysates were prepared for Western blot analysis.
