Fig. 7: OTUD7B depletion inhibits ERα-positive breast cancer cell proliferation and migration.
From: OTUD7B stabilizes estrogen receptor α and promotes breast cancer cell proliferation
A OTUD7B depletion inhibited cell proliferation in breast cancer cells. B OTUD7B depletion induced G1 cell cycle arrest in breast cancer cells. C OTUD7B depletion decreased clone formation capability of breast cancer cells. D, E Representative images of EdU assay of breast cancer cells. F Wound-healing assay of breast cancer cells. MCF-7- and T47D cells were transfected with indicated 50 nM OTUD7B siRNA or 50 nM control siRNA. Twenty four hours after transfection, cells were seeded into 6-well plates with 1% FBS with 100% confluence. A straight scratch was made on the cell layer with a yellow pipette tip. Quantification of wound closure was measured every 24 h, and the ERα protein level was measured at the endpoint. *P value < 0.05, **P value < 0.01, ***P value < 0.001.
