Fig. 8: Increased ERα expression reverses the effect of OTUD7B depletion.
From: OTUD7B stabilizes estrogen receptor α and promotes breast cancer cell proliferation
A Cell proliferation assay of MCF-7. B Clone formation assay of MCF-7. C Representative images of EdU assay of breast cancer cells. D Wound-healing assay of MCF-7. E Overexpression of ERα in OTUD7B-knockdown cells recovered tumor growth in vivo. F In total 2 × 106 MCF-7 cells were injected into the mammary fat pad of female BALB/c nude mice. When the tumor had grown to an appropriate volume (200 mm3), the tumor-bearing mice were randomly divided into groups. Cholesterol-modified OTUD7B siRNA or control siRNA (Ribobio, 10 nmol/kg) dissolved in diluted water were intratumorally injected every 3 days until the end of the experiment. *P value < 0.05, **P value < 0.01, ***P value < 0.001.
