Fig. 4: Let-7f, SDF-1α, hypoxia, and autophagy stimulate the exosomal release of let-7f. | Cell Death & Disease

Fig. 4: Let-7f, SDF-1α, hypoxia, and autophagy stimulate the exosomal release of let-7f.

From: Let-7f miRNA regulates SDF-1α- and hypoxia-promoted migration of mesenchymal stem cells and attenuates mammary tumor growth upon exosomal release

Fig. 4

A–C hMSCs were transfected with let-7f mimics or control oligonucleotides and/or incubated for 24 h in the absence (untreated) or presence of an inhibitor of exosome formation, GW4869 (10 µM), or SDF-1α (100 ng/mL), hypoxia (1% O2), and an inducer of autophagy, rapamycin (5 µM). qRT-PCR analysis of absolute let-7f expression in hMSCs upon normalization against snoRNA. A and in culture supernatants (Super) as well as exosomes (Exo) isolated thereof (B, C). A–C Results are given as mean ± SD of triplicate experiments (n = 3); ***P < 0.001, **P < 0.01.

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