Fig. 6: TFRC is a direct downstream target of MYCN.
From: MYCN mediates TFRC-dependent ferroptosis and reveals vulnerabilities in neuroblastoma
A RT-qPCR analysis of SK-N-BE2 cells with or without MYCN depletion. MYCN activation induced by 4-hydroxytamoxifen (4-HT) treatment promotes TFRC expression at both mRNA B and protein C levels. SKP2, DKK3 were used as positive and negative controls, respectively. D Amounts of cellular lipid peroxides measured by C11-BODIPY upon MYCN activation. E Primers designed for ChIP-qPCR analysis, and sequence variants for luciferase assays based on the TFRC promoter region. F ChIP-qPCR analysis of different binding sites of MYCN on the TFRC promoter. MDM2 is a positive control for MYCN binding. G Dual-luciferase assays based on (E). Signal values were normalized to no 4-HT treatment (day 0). Data are presented as mean ± SD of three replicates. Two-tailed unpaired t-tests were performed to calculate p values. **p < 0.01.
