Fig. 5: Dual modulation effects of oxidative stress and AMPK (NAC + AICAR) suppresses apoptosis and senescence (2 + 2DG) in chondrocytes. | Cell Death & Disease

Fig. 5: Dual modulation effects of oxidative stress and AMPK (NAC + AICAR) suppresses apoptosis and senescence (2 + 2DG) in chondrocytes.

From: High glucose suppresses autophagy through the AMPK pathway while it induces autophagy via oxidative stress in chondrocytes

Fig. 5

A Western blot results and quantification analyses of p62 and LC3 in various treatment groups. B Western blot results and quantification analyses of p53, p21, and p16 in various treatment groups. C Representative micrographs of SA‐β‐gal staining of chondrocytes in various treatment groups. Scale bar = 50 μM. D Western blot results and quantification analyses of Bcl2, Bax, and cleaved caspase-3 in various treatment groups. E Representative micrographs of TUNEL staining of chondrocytes to evaluate effects on apoptosis in various treatment groups. Scale bar= 100 μM. F TUNEL-positive cell intensity in various treatment groups. Data are presented as means ± SD (n = 5). *P < 0.05, **P < 0.01, and ***P < 0.001.

Back to article page