Fig. 3: SP1 was required in circ_0026628-mediated biological functions of CRC cells. | Cell Death & Disease

Fig. 3: SP1 was required in circ_0026628-mediated biological functions of CRC cells.

From: Hsa_circ_0026628 promotes the development of colorectal cancer by targeting SP1 to activate the Wnt/β-catenin pathway

Fig. 3: SP1 was required in circ_0026628-mediated biological functions of CRC cells.

A qRT-PCR and western blot measured the influence of silenced circ_0026628 on SP1 expression in CRC cells. One-way ANOVA. B–D. Colony formation, transwell (scale bar: 180 μm) and wound-healing assays (scale bar: 120 μm) examined the rescue effects of SP1 overexpression on circ_0026628 deficiency hampered cell proliferation and migration. One-way ANOVA. E, F Western blotting and IF staining assay (scale bar: 150 μm) detected changes in EMT process in indicated CRC cells. G, H Western blot and sphere formation assay (scale bar: 150 μm) explored the stemness of CRC cells under different transfections. One-way ANOVA. **P < 0.01.

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