Fig. 1: KDM4A is essential for skeletal muscle development.
From: KDM4A regulates myogenesis by demethylating H3K9me3 of myogenic regulatory factors
a Outline of the scheme to obtain control and KDM4A cKO mice. b Representative images of control and KDM4A cKO mice in 4 months. Scale bar = 1 cm. c The body weight of control and KDM4A cKO mice (4 months of age, n = 11). d Representative image of tibialis anterior (TA) muscle from control and KDM4A cKO mice at 4 months of age. e Quantification of TA weight/body weight in control and KDM4A cKO mice shown in (d) (n = 12, each). f Hematoxylin and eosin (H&E) staining of the TA muscle cross-sections from control and KDM4A cKO mice in 4 months. Scale bar = 100 μm. g Distribution of myofiber diameters of TA muscles from control and KDM4A cKO mice as described in (f) (n = 4, each). h Immunofluorescent staining for laminin and nuclei (DAPI) on TA muscle cross-sections of control and KDM4A cKO mice (5 months of age). Scale bar = 100 μm. i The distribution of fiber size measured by CSA in TA muscles from control and KDM4A cKO mice (n = 4, each). j qRT-PCR detection of the KDM4A and myogenic genes expression in the TA muscles from 4-months old control or KDM4A cKO mice (n = 4). k Protein levels of genes expression indicated in (j). l Quantifications of embryos weight from control or KDM4A cKO mice at E17.5 (n = 9). m qRT-PCR analysis showing relative transcript levels of indicated genes from E17.5 embryos. n Immunofluorescence analysis of eMyHC+ fibers in dorsal muscles of control and KDM4A cKO embryos at E17.5. Scale bar = 100 μm. o, p Quantifications of average myofiber CSA and the numbers of MyHC+ fibers per area in (n). Data are represented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; n.s. not significant (Student’s t test).
