Fig. 6: HBO1 KO inhibits HCC xenograft growth in SCID mice.
From: HBO1 overexpression is important for hepatocellular carcinoma cell growth
Primary HCC1 cells, bearing CRISPR/Cas9 HBO1-KO construct (“KO-HBO1”) or the empty vector (“Cas9-C vector”), were s.c. inoculated to the SCID mice (with 11 mice per group). Recordings were started when the volume of each tumor close to 100 mm3 (“Day-0”). Tumor volumes (A) and mice body weights (D) were recorded every seven days for a total of 35 days; Estimated daily tumor growth was calculated as described (B); At Day-35 all tumors were isolated and weighted individually (C). The tumor lysates were subjected to qPCR (E) and Western blotting (F) assays. Error bars indicate mean ± standard deviation (SD, n = 5). *P < 0.05 vs. “Cas9-C vector” tumors.
