Fig. 2: Erythropoiesis defects in Gata2^L359V/L359V embryos.

A Morphological analysis of Gata2^WT/WT (upper panel) and Gata2^L359V/L359V (lower panel) yolk sac erythrocytes by using Wright’s staining (scale bar 10 μm). B Immunophenotype of E10.5 yolk sac cells marked by CD71/Ter119 and the cells were divided into five stages (R1-R5) following the expression levels of CD71 and Ter119. C Statistical analysis of the percentage of CD71^lowTer119^low, CD71^highTer119^low, and CD71^highTer119^high cells between Gata2^WT/WT (n = 3) and Gata2^L359V/L359V (n = 3). D Principal component analysis (PCA) of the RNA-seq data of the Gata2^WT/WT, Gata2^WT/L359V, and Gata2^L359V/L359V yolk sacs at E9.5. E Heatmap showing a group of genes uniquely down-regulated or upregulated in Gata2^L359V/L359V as compared to Gata2^WT/L359V and Gata2^WT/WT E9.5 yolk sacs. F Gene ontology(GO) analysis of embryonic development genes that were uniquely up/down-regulated in Gata2^L359V/L359V as compared to Gata2^WT/L359V and Gata2^WT/WT E9.5 yolk sacs. G The fragments per kilobase million (FPKM) value of representative genes upregulated or down-regulated in Gata2^L359V/L359V E9.5 yolk sacs (n = 3, respectively). Error bars represent the standard deviation from the average. Significant differences are indicated by *p < 0.01 (Student’s t test).