Fig. 4: Expression of miR-23b-3p and miR-29a-3p are essential for PF formation in the perinatal mouse ovaries.

a qRT-PCR analysis of miR-23b-3p (top) and miR-29a-3p (bottom) expression at the indicated time points. Gapdh was used to normalize samples. b RNA-FISH analysis of miR-23b-3p and miR-29a-3p expression in perinatal mouse ovaries from 18.5 dpc, and 0.5 dpp. The nucleus was stained by DAPI (blue). Dashed circle indicating representative cysts. Arrows heads indicating oocytes. Scale bars: 5 μm. c, d qRT-PCR analysis of miR-23b-3p (top) and miR-29a-3p (bottom) expression in newborn ovaries transfected with anti-miR-23b-3p, anti-miR-29a-3p, or anti-miR-NC (c), and mimics for miR-23b-3p, miR-29a-3p, or miR-NC (d). e, f Representative DDX4 immunofluorescence images (left) and quantification of follicles (right) in newborn ovaries transfected with anti-miR-23b-3p, anti-miR-29a-3p, or anti-miR-NC (e), and mimics for miR-23b-3p, miR-29a-3p, or miR-NC (f). Scale bars: 50 μm. g–j qRT-PCR analysis of genes involved in PF survival and development in newborn ovaries transfected with anti-miR-23b-3p (g), anti-miR-29a-3p (h), or anti-miR-NC, and mimics for miR-23b-3p (i), miR-29a-3p (j), or miR-NC. Student’s t-test: *P < 0.05, **P < 0.01, ***P < 0.001.