Fig. 5: STX17 is a downstream target of Xist-miR-23b-3p/miR-29a-3p axis and regulates PF formation. | Cell Death & Disease

Fig. 5: STX17 is a downstream target of Xist-miR-23b-3p/miR-29a-3p axis and regulates PF formation.

From: Long non-coding RNA Xist regulates oocyte loss via suppressing miR-23b-3p/miR-29a-3p maturation and upregulating STX17 in perinatal mouse ovaries

Fig. 5: STX17 is a downstream target of Xist-miR-23b-3p/miR-29a-3p axis and regulates PF formation.The alternative text for this image may have been generated using AI.

a The Venn diagram shows overlapping of the co-target genes of miR-23b-3p/miR-29a-3p from five representative databases. b The predicted binding sites of miR-23b-3p and miR-29a-3p binding sites in the 3′-UTR of Stx17. The red nucleotides represent mutant sequences of target sites. c Luciferase activities in HEK293 cells transfected with WT or mutant Stx17 plasmid together with miR-23b-3p or miR-29a-3p, or miR-NC. d Enrichment of Stx17 pulled down by biotin-miR-23b-3p, biotin-miR-29a-3, or biotin-miR-NC control in 3T3 cells. e–h Relative Stx17 mRNA (left), and STX17 protein (right) expression in cultured newborn ovaries transfected with mimics for miR-23b-3p, miR-29a-3p, or miR-NC (e), anti-miR-23b-3p, anti-miR-29a-3p, or anti-miR-NC (f), Xist or empty control (g), and siXist or scrambled control (h). i Relative Stx17 mRNA (left), and STX17 protein (right) expression at the indicated time points. j Relative Stx17 mRNA (left), and STX17 protein (right) expression in cultured newborn ovaries transfected with siStx17, or siNC as a control. k Representative images (left) and quantification of follicles (right) in newborn ovaries transfected with siStx17, or siNC. Scale bars: 50 μm. l qRT-PCR analysis of genes involved in PF survival and development in newborn ovaries transfected with siStx17, and siNC as a control. m WB analysis of LC3B expression in newborn ovaries treated under indicated condition. n Representative images of DDX4 in newborn ovaries co-transfected with pcDNA-Xist or empty vector together with siStx17 or scrambled control. Scale bars: 50 μm. o Quantification of follicles in n. p WB analysis of STX17 expression in newborn ovaries treated under indicated condition. GAPDH was used as a loading control. Student’s t-test: *P < 0.05, **P < 0.01, ***P < 0.001.

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