Fig. 6: YY1 is essential for Xist expression and functions as an upstream regulator for Xist-miR-23b-3p/miR-29a-3p-Stx17 axis during PF formation. | Cell Death & Disease

Fig. 6: YY1 is essential for Xist expression and functions as an upstream regulator for Xist-miR-23b-3p/miR-29a-3p-Stx17 axis during PF formation.

From: Long non-coding RNA Xist regulates oocyte loss via suppressing miR-23b-3p/miR-29a-3p maturation and upregulating STX17 in perinatal mouse ovaries

Fig. 6: YY1 is essential for Xist expression and functions as an upstream regulator for Xist-miR-23b-3p/miR-29a-3p-Stx17 axis during PF formation.

a Schematic representation for YY1 binding motif within the proximal promoter region of Xist, with respective primer sets to amplify YY1 binding region. The upstream control region is also represented (top). Bottom: ChIP analysis of YY1 binding in newborn mouse ovaries at 0.5 dpp, and 4.5 dpp. The dashed line represents the basal IgG binding. b, c Relative Yy1 mRNA (b), and YY1 protein (c) expression in perinatal ovaries at indicted time. d, e Relative Yy1 mRNA (top), and YY1 protein (bottom) expression in cultured newborn ovaries transfected with siYy1 or control (d), pcDNA3.1-Yy1, and NC control (e). f Relative Xist mRNA expression in cultured newborn ovaries transfected with siYy1 or siRNA control (top), pcDNA3.1-Yy1 or NC control (bottom). g, h Representative DDX4 immunofluorescence images (g) and quantification of follicles (h) in newborn ovaries transfected under indicated condition. Scale bars: 50 μm. i WB analysis of STX17 expression in newborn ovaries treated under indicated condition. j Proposed model for Xist-miR-23b-3p/miR-29a-3p-STX17 axis in regulating oocyte loss in the perinatal mouse ovaries. Student’s t-test: *P < 0.05, **P < 0.01, ***P < 0.001.

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