Fig. 3: CPX induces apoptosis in bladder cancer cell lines.
From: Fosciclopirox suppresses growth of high-grade urothelial cancer by targeting the γ-secretase complex
A T24 and UM-UC-3 cells were treated with CPX stained with Annexin V (FITC) and PI, and analyzed by flow cytometry. CPX treatment induced significant early and late apoptosis in T24 and UM-UC-3 cells. B The flow cytometric quantification of early and late apoptotic cells after CPX treatment over a period of 72 h in T24 and UM-UC-3 cells in Annexin-PI assay. C CPX induces apoptosis in T24 cells. T24 cells treated with CPX (4 µM) for 48 h were stained with Annexin V-FITC/PI solution and studied using immunofluorescence. CPX induced apoptosis in T24 cells. D Lysates from T24 and UM-UC-3 cells treated with CPX were studied by western blot for evaluating the effect on proteins involved in apoptosis. CPX treatment reduced the levels of antiapoptotic marker proteins Bcl2 and Bcl-XL. CPX treatment also increases the PARP cleavage compared to untreated controls. E CPX induces autophagy early which then followed by apoptosis. Lysates from T24 and UM-UC-3 cell treated with CPX (4 µM) for 24–48 h were analyzed by western blot. CPX treatment increased the expression of LC3B at 24 h and cleaved caspase-3 expression at 48 h. The data suggest that at early time points, CPX induces autophagy, which switches to apoptosis in T24 and UM-UC-3 cells.
