Fig. 4: CPX inhibits bladder cancer spheroid growth and cell migration.
From: Fosciclopirox suppresses growth of high-grade urothelial cancer by targeting the γ-secretase complex
A, B CPX inhibits spheroid growth. CPX suppressed the size (A) and number (B) of spheroids of bladder cancer cell lines. Cells were grown in spheroid growth media in ultralow adherent plates and treated with CPX 2 µM and 4 µM concentrations for 5 days. CPX significantly decreased bladdosphere formation in the T24, UM-UC-3, HT-1376, HTB-9, and RT-4 cell lines. C CPX inhibits the expression of bladder cancer stem cell marker proteins. Lysates from T24 and UM-UC-3 cell lines after CPX treatment were subjected to western blot analysis. CPX treatment inhibited the expression of SOX9 and CD44 in both cell lines. D CPX inhibits migration of T24 and UM-UC-3 cells. Both cell lines were grown to 95–100% confluency and a scratch was made to study the cell movement over a period of 12 h. CPX treatment reduced cell migration. E CPX inhibited the percent migration in both cell lines. F CPX inhibits migration of T24 and UM-UC-3 cells. CPX treatment significantly reduced the migration of both cell lines through transwell cell culture inserts (Boyden’s chamber). G The percent migraition is represented in graphical format.
