Fig. 5: Nissl staining was used to detect the effect of Gabrg2 deletion on hippocampus and cortical neurons.

a Comparison of the position and mapping of the left hemisphere. On the right, a representative Nissl image from three independent experiments of the mice brain (coronal section, −1.8 mm relative to the bregma) shows the stained neurons of WT mice. Scale bar: 1 mm. b Representative Nissl staining of the hippocampus and cerebral cortex of WT mice. These neurons are neat, relatively close, and have a lot of synapses. In WT mice, layer V (deep Pyramidal) contains the largest pyramidal neurons of the cortex, which project their axons to a variety of cortical and sub-cortical targets. Scale bar: 300 µm. c On the sections of the hippocampus’ CA1, CA3 and DG regions, and neocortex in Gabrg2^fl/wtCre⁺ mice, there is a decrease in the number of neurons, and cells are arranged disorderly. In layer V of the neocortex, the pyramidal neurons were significantly less in the Gabrg2^fl/wtCre⁺ mice than that of the WT mice. Scale bar: 300 µm. d Histograms of the Nissl body counts in the hippocampus quantified from Nissl staining analysis in WT (n = 4) and Gabrg2^fl/wtCre⁺ mice (n = 6). e The number of Nissl body was counted in the neocortex in two group (n = 4 for WT and n = 6 for KO). Black arrow: Nissl body. Data shown are mean ± standard error of mean. ***P < 0.001 and ****P < 0.0001 vs WT, t-test (two-tailed).