Fig. 6: Seizures induced by temperature rising and abnormal electroencephalography (EEG) activity in Gabrg2^fl/wtCre⁺ conditional knockout mice.

a Photograph of the temperature controller and heating setup. b Percentage of myoclonic jerks and/or generalised tonic-clonic seizures (GTCS) was plotted for the adult Gabrg2^fl/wtCre⁺ and WT mice with rising body temperature. For adult Gabrg2^fl/wtCre⁺, more than 80% of mice have temperature-induced seizures at 39.5 °C; however, WT mice did not. c The rate of core body temperature changes in adult Gabrg2^fl/wtCre⁺ and WT mice was measured every 2 min during the heating process. The change rate of core temperature per 2 min was higher in Gabrg2^fl/wtCre⁺ (n = 21) than in WT (n = 24) mice (P < 0.01). d Representative 1 s traces of intracranial EEG activity for WT mice during temperature elevation until 42.5 °C. There was no seizure above stage 3 observed in any WT mouse during heating process (n = 24). e Representative intracranial EEG traces of Gabrg2^fl/wtCre⁺ mice during temperature-induced seizures. With the temperature rising, the higher spikes and amplitudes were seen. f The GTCSs was induced at 39 °C. Data shown are mean ± standard error of mean. **P < 0.01 vs WT, t-test (two-tailed).