Fig. 7: Temperature- and pentylenetetrazole (PTZ)-induced seizure latency, severity, as well as survival between Gabrg2^fl/wtCre⁺ and wild type mice.

a The modified Racine grade was used to evaluate the scores of seizure severity between Gabrg2^fl/wtCre⁺ (n = 21) and WT (n = 24) mice during the heating process. The severity of seizures increased markedly in Gabrg2^fl/wtCre⁺ mice compared to WT mice. b A value of 100% was allocated to the average seizure severity score of the WT mice, and then applied to normalise the severity of the Gabrg2^fl/wtCre⁺ mice within the same scale. The seizure severity was significantly higher in Gabrg2^fl/wtCre⁺ mice compared to WT mice during temperature-induced seizure. c The latency to reach stages 2/3 of the modified Racine scale was significantly different between Gabrg2^fl/wtCre⁺ and WT mice for temperature-induced seizures. d Survival percentage indicated that there was no significant difference (0 died for WT vs. 0 died for Gabrg2^fl/wtCre⁺, P > 0.05) in survival between Gabrg2^fl/wtCre⁺ and WT mice over 30 min during temperature-induced seizure process. e The modified Racine grade was used to evaluate the scores of seizure severity between Gabrg2^fl/wtCre⁺ (n = 21) and WT (n = 24) mice during PTZ-induced seizures. The severity of seizure increased significantly in Gabrg2^fl/wtCre⁺ mice as compared with WT mice. f The seizure severity was significantly higher in Gabrg2^fl/wtCre⁺ mice compared with WT mice for PTZ- induced seizure. g The latency to reach stages 2/3 of the modified Racine scale was significantly different between Gabrg2^fl/wtCre⁺ and WT mice for PTZ-induced seizures. h The mortality increases significantly in Gabrg2^fl/wtCre⁺ mice compared with WT mice after intraperitoneal injection of PTZ (11/21 died for Gabrg2^fl/wtCre⁺ vs. 1/24 died for WT). Data shown are mean ± standard error of mean. **P < 0.01 and ***P < 0.001 vs WT, t-test (two-tailed).