Fig. 3: BMS309403 decreased the expression of fibrosis marker in TGF-β-treated HK-2 cells via inhibiting TGF-β/Smad pathway. | Cell Death & Disease

Fig. 3: BMS309403 decreased the expression of fibrosis marker in TGF-β-treated HK-2 cells via inhibiting TGF-β/Smad pathway.

From: Pre-emptive pharmacological inhibition of fatty acid–binding protein 4 attenuates kidney fibrosis by reprogramming tubular lipid metabolism

Fig. 3

a Western blot of HK-2 cell lysates for FABP4, fibronectin, collagen-I, and α-SMA expression. GAPDH sets as loading control. b RT-qPCR for fibronectin, collagen-I, and α-SMA transcripts normalized to Gapdh in HK-2 cells. c Western blot and band intensity quantitation for TGF-β, p-Smad2, and p-Smad3 in HK-2 cells. Blots were stripped and reprobed for GAPDH. d RT-qPCR for TGF-β, CTGF, FGF2, PDGFB transcripts normalized to Gapdh in total RNA extracts of HK-2 cells from DMSO- or BMS309403-treated normal and TGF-β-treated cells. These data were calculated from three independent experiments. ***P < 0.001.

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