Fig. 6: Endothelial cell apoptosis and inflammation were alleviated in a KD mouse model after IL-37b treatment. | Cell Death & Disease

Fig. 6: Endothelial cell apoptosis and inflammation were alleviated in a KD mouse model after IL-37b treatment.

From: IL-37b alleviates endothelial cell apoptosis and inflammation in Kawasaki disease through IL-1R8 pathway

Fig. 6: Endothelial cell apoptosis and inflammation were alleviated in a KD mouse model after IL-37b treatment.The alternative text for this image may have been generated using AI.

a, b The protein expression of BAX and Bcl-2 was examined in the KD mouse model after IL-37b treatment (n = 6). c Effects of IL-37b treatment on DNA fragmentation in the endothelial cells were evaluated by co-localized staining of TUNEL (green) and CD31 (an endothelial marker, red) (n = 5). Magnification: ×200. Scale bar = 50 μm. d TNF-α expression levels were examined in the coronary artery endothelial cells by double staining of TNF-α (green) and CD31 (red) (n = 5). Magnification: ×200. Scale bar = 50 μm. e–g The activation of ERK and NFκB was determined in the heart tissues by western blot analysis (n = 6). h The levels of p-ERK were examined in the coronary artery endothelial cells using p-ERK/CD31 double staining (n = 5). Magnification: ×200. Scale bar = 50 μm. Significance: *P < 0.05 vs. the PBS group, and #P < 0.05 vs. the CAWS group. Each experiment was conducted at least three times.

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