Fig. 6: Treatment of CXCR2 antagonist suppresses low SLC7A2-mediated HCC metastasis. | Cell Death & Disease

Fig. 6: Treatment of CXCR2 antagonist suppresses low SLC7A2-mediated HCC metastasis.

From: SLC7A2 deficiency promotes hepatocellular carcinoma progression by enhancing recruitment of myeloid-derived suppressors cells

Fig. 6

A, B Conditioned media (CM) from Hepa1-6 clones was placed in the lower chambers with different concentration of SB265610 (0, 100,1000 ng/mL, 24 h). Freshly Myeloid MDSCs were put in the upper chambers and invasion for 24 h. Total numbers were counted. The scale bars represent 100 μm. The data are shown as the mean ± SD from the at least three independent experiments; *P < 0.05, **P < 0.01. C Pre-treated with mCXCL1 (10 ng/mL, 24 h) and SB265610 (100 ng/mL, 24 h), the chemotaxis index of mouse MDSCs is shown. The data are shown as the mean ± SD from the at least three independent experiments; *P < 0.05. D Tumor growth in mice subcutaneously injected with Hepa1-6-shcontrol or Hepa1-6-shSLC7A2 cells, treated with CXCR2 antagonist (SB265610: 2 mg/kg body weight) or PBS six times a week from day 1 after tumor inoculation. n = 10. E Flow cytometric images of subcutaneous Hepa1-6-shcontrol or Hepa1-6-shSLC7A2 cells treated with SB265610 or PBS at day 27. CD8+ T cells (upper) and MDSCs (lower). Flow cytometric analyses the ratio of CD8+ T cells and MDSCs between groups at day 27 respectively, n = 10. The data are shown as the mean ± SD from the at least three independent experiments; *P < 0.05, **P < 0.01, and ***P < 0.001. F Immunofluorescent staining of the CD11b, CD8, and granzyme B protein expression patterns in mice tumor cells. G–K In vivo assays shown that the treatment of SB265610 can block knock-down SLC7A2-mediated HCC metastasis. G The representative Bioluminescence images were shown in the different groups, treated with SB265610 or PBS in the C57BL/6 injected with the indicated cells in the liver, n = 10. The Bioluminescence intensity in the tumors at the indicated time point was presented as the total photon flux. H Incidence of lung metastasis I the number of metastatic lung nodules in lung in the C57BL/6 mice. n = 10. J Overall survival of the mice in each group. K Representative H&E-stained lung metastatic nodules. The scale bars represent 1 mm (upper panel) and 100 μm (lower panel). Images shown are representative of at least three independent experiments. *P < 0.05, **P < 0.01.

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