Fig. 5: The role of KMT5A in promoting proliferation of PA cells was regulated by miR-502-3p. | Cell Death & Disease

Fig. 5: The role of KMT5A in promoting proliferation of PA cells was regulated by miR-502-3p.

From: LncRNA LINC00473 is involved in the progression of invasive pituitary adenoma by upregulating KMT5A via ceRNA-mediated miR-502-3p evasion

Fig. 5: The role of KMT5A in promoting proliferation of PA cells was regulated by miR-502-3p.The alternative text for this image may have been generated using AI.

qRT-PCR detection of KMT5A mRNA expression in PA cells AtT-20 and GT1-1 after transfection of miR-502-3p mimic (a) and inhibitor (b). c Prediction of binding sites for KMT5A and miR-502-3p, wide type and mutant type of KMT5A were designed for luciferase reporter assay. d Dual luciferase reporter gene assay was used to detect the binding ability between KMT5A and miR-502-3p. e CCK-8 detection of PA cells viability after overexpression of KMT5A, knockdown of KMT5A, or knockdown of KMT5A while inhibition of miR-502-3p. f Cell proliferation was determined by EdU staining. g Western blot analysis of cyclin D1, CDK2, and KMT5A expression. h Cell cycle was analyzed by flow cytometry. *P < 0.05, **P < 0.01, ***P < 0.001, data represent the mean ± SD.

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