Fig. 1: ECCA selectively blocks the growth of melanoma cells compared to human primary melanocytes. | Cell Death & Disease

Fig. 1: ECCA selectively blocks the growth of melanoma cells compared to human primary melanocytes.

From: A carbazole compound, 9-ethyl-9H-carbazole-3-carbaldehyde, plays an antitumor function through reactivation of the p53 pathway in human melanoma cells

Fig. 1

A Five melanoma cell lines were treated with different concentrations of ECCA as indicated, with DMSO used as a control. At 48 h, cells were collected and analyzed by the CCK8 assay for cell viability. B UACC62 cells were treated with different concentrations of ECCA as indicated, with DMSO as a control. Cells were collected at the different time points indicated and were analyzed by the CCK8 assay for cell viability. C UACC62 cells and human primary melanocytes were treated with or without various concentrations of ECCA as shown. At 48 h, cells were collected and analyzed by the CCK8 assay for cell viability. D Representative images of cell colony formation assays with or without different concentrations of ECCA as indicated at 7 days using crystal violet staining. Scale bar represents 5 mm. E Quantification of cell colony numbers in D. F Representative images of immunofluorescence staining of EDU (green) at 24 h after ECCA treatment at different concentrations as indicated. DAPI (blue) stain indicates nuclei. White arrows indicate EDU-positive cells. Scale bars represent 100 μm. G Quantification of EDU-positive cell percentage (%) of a total of 500 DAPI-positive cells in F. All experiments were carried out three times, and error bars represent means + SD; P values are indicated with “*”, * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.005 when comparing ECCA-treated cells with the control group in A, B, E, and G by Student’s t test and comparing two groups as indicated in C by ANOVA assay.

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