Fig. 7: Pirh2 activates H1299 cell proliferation and cooperates with c-Myc in lung cancer progression.
From: The RNA-binding protein HuR is a novel target of Pirh2 E3 ubiquitin ligase

A Pirh2 stimulates proliferation of H1299 cells. Cell index graphs demonstrating the proliferation registered by xCelligence system for H1299 cells with stable Pirh2 overexpression (Pirh2 OE) and control H1299 cells (ctrl). B Pirh2 knockdown suppresses proliferation of H1299 cells. Cell index graphs registered by xCelligence system for H1299 cells with stable Pirh2 knockdown (Pirh2 KD) and control H1299 cells (scrambled). C The bioinformatics analysis of the effect of simultaneous rchy1 and myc genes (coding Pirh2 and c-Myc, respectively), and elavl1 gene (coding HuR protein) expression on lung adenocarcinoma patients’ (GEO dataset ID: GSE31210) survival probability. P-values are indicated. D Graphical scheme summarizing the role of Pirh2 in HuR protein stability regulation. Pirh2 ubiquitinates HuR that leads to HuR proteasomal degradation and influence on HuR target mRNA stability.