Fig. 5: Prrx1 promotes stemness maintenance and angiogenesis, as well as activates TGF-β/smad pathway in GSCs. | Cell Death & Disease

Fig. 5: Prrx1 promotes stemness maintenance and angiogenesis, as well as activates TGF-β/smad pathway in GSCs.

From: Prrx1 promotes stemness and angiogenesis via activating TGF-β/smad pathway and upregulating proangiogenic factors in glioma

Fig. 5: Prrx1 promotes stemness maintenance and angiogenesis, as well as activates TGF-β/smad pathway in GSCs.

A Western blot analysis of the expression of GSC markers and TGF-β/smad pathway markers in Prrx1 overexpression and knockdown primary GSCs. B, C Sphere formation assay (B) and colony formation assay (C) of GSCs to explore the effect of Prrx1 on stemness maintenance. Scale bar represents 50 μm. D Representative capillary tubule structures were shown for HUVECs treated with culture medium collected from the indicated primary GSCs. Scale bar represents 50 μm. E Blood vessels formed in representative images of the CAM assay after CM treatment. F Transwell assay was performed in HUVECs to detect the effect of CM treatment on cell migration. Scale bar represents 50 μm. *P < 0.05, **P < 0.01.

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