Fig. 5: PRDX2 increases MDM2-induced p53 ubiquitination. | Cell Death & Disease

Fig. 5: PRDX2 increases MDM2-induced p53 ubiquitination.

From: PRDX2 promotes the proliferation of colorectal cancer cells by increasing the ubiquitinated degradation of p53

Fig. 5: PRDX2 increases MDM2-induced p53 ubiquitination.

A The degradation of p53 depends on the activation of the proteasome. Cells in the NC and shPRDX2 groups were treated with MG132 (20 µM) for 6 h; p53 and PRDX2 expression was measured by western blotting. C, E Total ubiquitinated protein levels in the NC and shPRDX2 groups were measured by western blotting. D, F PRDX2 increased the ubiquitin level of p53. Whole-cell lysates were immunoprecipitated with an anti-p53 antibody; the immunocomplexes were examined by WB with an anti-Ub antibody, and whole-cell lysates were subjected to western blot analysis with the indicated antibodies. B p53 ubiquitination was MDM2-dependent. The cells in the NC and shPRDX2 groups were transfected with siMDM2, and the expression of the indicated protein was measured by western blotting.

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