Fig. 7: ISL1 promoted EMT in NB cell lines through PI3K/AKT signal pathway. | Cell Death & Disease

Fig. 7: ISL1 promoted EMT in NB cell lines through PI3K/AKT signal pathway.

From: ISL1 promoted tumorigenesis and EMT via Aurora kinase A-induced activation of PI3K/AKT signaling pathway in neuroblastoma

Fig. 7

A, B Expression changes of EMT-associated markers (epithelial marker: E-cadherin, mesenchymal markers: N-cadherin, vimentin, and Slug) in SK-N-SH and SK-N-BE (2) cells after ISL1 was downregulated by si-ISL1-2. C, D WB analysis of total AKT and p-AKT in SK-N-SH and SK-N-BE (2) cell lines after transfection with si-ISL1-2. E Apoptosis analysis of SK-N-SH and SK-N-BE (2) cell lines treated with PI3K inhibitor LY294002 for 48 h. F Comparison of ISL1, AKT, p-AKT protein, and EMT-associated markers’ expression in SK-N-SH and SK-N-BE (2) cells with or without using PI3K inhibitor LY294002 (concentration at 0, 2.5, 5, and 10 μM. *P < 0.05, **P < 0.01, ***P < 0.001).

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