Fig. 3: SIRT4-OE restrained cell survival and enhanced intracellular ROS. | Cell Death & Disease

Fig. 3: SIRT4-OE restrained cell survival and enhanced intracellular ROS.

From: VHL regulates the sensitivity of clear cell renal cell carcinoma to SIRT4-mediated metabolic stress via HIF-1α/HO-1 pathway

Fig. 3: SIRT4-OE restrained cell survival and enhanced intracellular ROS.

A KEGG pathway enrichment analysis based on SIRT4 association genes was performed by Metascape. B The efficiency of SIRT4 overexpression and SIRT4-H161Y reconstitution in Caki-2 and 786-O cells were validated by western blot. C Effects of SIRT4 or SIRT4-H161Y overexpression on cell growth was monitored by CCK8 (left panel) and representative phase-contrast photomicrograph (middle panel). The quantification analysis is shown as the right panel. D The effects of SIRT4-OE and H161Y reconstitution on colony formation capacity. Caki-2 and 786-O cells were cultured in a complete medium (CM) with or without supplemental α-KG (25 μM). A summary of the quantification analysis is shown underneath. E NADPH levels in cells with SIRT4 overexpression and H161Y reconstitution. F Intracellular ROS monitored by the DCFH-DA fluorescent probe (DCF) via FACS. Data are expressed as mean ± SD, experiments were done in three independent times. Throughout the figure, *P < 0.05, **P < 0.01, ns: no significance.

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