Fig. 4: SIRT4-OE induced apoptosis by ROS enhancement. | Cell Death & Disease

Fig. 4: SIRT4-OE induced apoptosis by ROS enhancement.

From: VHL regulates the sensitivity of clear cell renal cell carcinoma to SIRT4-mediated metabolic stress via HIF-1α/HO-1 pathway

Fig. 4: SIRT4-OE induced apoptosis by ROS enhancement.

A The effect of SIRT4-OE and H161Y reconstitution on apoptosis was evaluated in Caki-2 and 786-O cells. Apoptosis (cells with annexin V positive) ratio was revealed as right. B Clustering heatmaps were performed based on the expression of SIRT4 and apoptosis-related genes (BCL2, CASP8, CASP9, CASP3, BAX) from the TCGA database. C Expression differences of CASP9 (caspase-9) and BAX between SIRT4-low and SIR4-high-expression subgroups based on the TCGA database (Nature, 2013). Data are plotted as means ± SD. D Protein expression levels of caspase-9 and Bax in Caki-2 and 786-O cells with SIRT4 overexpression or H161Y reconstitution. Densitometric analysis of western blots was exhibited underneath. After treating cells with NAC at a dose of 5 mM for 24 h, intracellular ROS levels were determined by DCFDA (DCF) staining (E), and apoptosis was determined by FACS (F). G Assess the effects of SIRT4 overexpression and NAC on protein caspase-9 expression by western blot. Densitometric analysis of caspase-9 is shown on the right panel. Throughout the figure, *P < 0.05, **P < 0.01, ***P < 0.001, ns: no significance.

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