Fig. 6: Clobetasol reverts denervation-induced mitochondrial fission and increases energy substrates.

a and b Representative pictures of MitoTracker immunostaining (a) and quantification of the mitochondrial length expressed in μm (b) in the left GM of HC, CTB-Sap vehicle, and CTB-Sap clobetasol mice; data in (b) are shown as scatter dot plot and mean ± SEM of n ≥ 3 mice per group; *p-value < 0.05, **p-value < 0.01 and ***p-value < 0.001 versus HC or between groups; one-way ANOVA; scale bar in (a): 25 μm. c mRNA expression levels of Dnm1l in the left GM of HC, CTB-Sap vehicle and CTB-Sap clobetasol mice; data are fold changes over HC and are shown as standard box-and-whiskers plot of n = 5 mice per group; *p-value < 0.05 versus HC or between groups; one-way ANOVA. d Heat maps of 24 metabolites in the left GM HC, CTB-Sap vehicle and CTB-Sap clobetasol at 42 dpl showing the abundance (Ab.) in HC and the relative changes in CTB-Sap vehicle and CTB-Sap clobetasol as compared to HC; data are shown as log₁₀ abundance and log₂ FC over HC of n = 4 mice per group. e and f Volcano plots of metabolites levels in HC versus CTB-Sap vehicle (e) and CTB-Sap vehicle versus CTB-Sap clobetasol (f). g Metabolites levels and ratios in the left GMs of HC, CTB-Sap vehicle, and CTB-Sap clobetasol mice; data are shown as mean ± SEM and scattered dots plot of n = 4 mice per group; *p-value < 0.05 and **p-value < 0.01 versus HC or between groups; one-way ANOVA.