Fig. 3: OGA associates with NONO and the Ku70/80 complex.
From: OGA is associated with deglycosylation of NONO and the KU complex during DNA damage repair
A The list of proteins that were identified from the HAT domain of OGA (C-OGA) affinity purification and mass spectrometry. B, C The HAT domain of OGA (C-OGA) interacts with exogenous and endogenous NONO and the Ku70/80 complex. Myc-NONO and SFB-C-OGA or SFB-N-OGA were expressed in 293T cells. When endogenous NONO and the Ku70/80 complex were examined, only N-OGA or C-OGA was expressed in 293T cells. Cells were lyzed with NETN300 buffer. Cell extracts were examined by IP and western blot with indicated antibodies. D, E The interactions between the HAT domain of OGA (C-OGA) and NONO or the Ku70/80 complex were increased following IR treatment. Indicated constructs were expressed in 293T cells, and the cells were treated with 10 Gy of IR. The cell extracts were examined by IP and Western blot with indicated antibodies. SFB vector stands for SBP-FLAG-S tandem tag vector. F The interactions between the HAT domain of OGA (C-OGA) or OGA-FL and endogenous NONO or the Ku70/80 complex. C-OGA or OGA-FL was expressed in 293T cells, and the cells were treated with or without 10 Gy of IR. The cell extracts were examined by IP and Western blot with indicated antibodies.
