Fig. 1: Characterization of circ_0008542. | Cell Death & Disease

Fig. 1: Characterization of circ_0008542.

From: Circ_0008542 in osteoblast exosomes promotes osteoclast-induced bone resorption through m6A methylation

Fig. 1

A A heat map of different circRNAs between the two groups with or without tension stimulation. B Bioinformatics prediction of the circ_0008542/miRNA-185-5p-RANK axis. C Schematic diagram showing the genomic location and back-splicing pattern of circ_0008542. D Sanger sequencing of circ_0008542. The arrow shows the “head-to-tail” splicing site. E Divergent primers detected circ_0008542 from cDNA by PCR and agarose gel electrophoresis, rather than from gDNA. F Circ_0008542 from cDNA was amplified with divergent primers, even after treatment with RNase R digestion, and the opposite results were observed for gDNA. G Relative expression level of circ_0008542 in exosomes between two groups with or without RNase R digestion. Data were representative of three independent experiments expressed as the mean ± SD (*p < 0.05).

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