Fig. 7: In vivo effect of TNF-α and schematic model of its action. | Cell Death & Disease

Fig. 7: In vivo effect of TNF-α and schematic model of its action.

From: TNF-α induces endothelial–mesenchymal transition promoting stromal development of pancreatic adenocarcinoma

Fig. 7

TNF-α increases CAFs in pancreatic tumour in vivo (A, B) and schematic model of the reversible TNF-α-induced EndMT involving Tie1 downregulation (C). A, B. TNF-α induces an increase of fibrosis (A) and an increase of CAFs α-SMA positives (B). PK4A tumoral cells were orthotopically injected in mice which were treated daily for 27 days with injections of NaCl (Ctrl) or TNF-α (n = 6 for each condition). Then, pancreases were collected and sections of tissue were subjected to HES staining, Masson’s trichrome (MT) staining and α-SMA staining. Fibrosis and α-SMA staining were analysed and quantified by calopix. Significant differences are indicated by solid lines (**P < 0.05, by t test). Scale bars, 100 µm. CAF cancer-associated fibroblast, TNF-α tumour necrosis factor-α, Ctrl control, HES haematoxylin eosin saffron, MT Masson’s trichrome MT, α-SMA α-smooth muscle actin.

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