Fig. 2: FAM64A promotes the proliferation, migration/invasion, and cell cycle of PCa cells.
From: FAM64A is an androgen receptor-regulated feedback tumor promoter in prostate cancer
a The mRNA and protein level of FAM64A in PCa cell lines LNCaP and 22Rv1 with transfection of siRNA-FAM64A was noteworthy lower than that in the control group. b, c CCK-8 assay and colony formation assay showed that knockdown of FAM64A decreased the proliferation of PCa cells. d Transwell invasion assay indicated that knockdown of FAM64A reduced the invasive activity of PCa cells. e, f Wound healing assay indicated that knockdown of FAM64A reduced the migratory ability of PCa cells at 0, 48, 96 h. g Flow cytometry with Annexin V-FITC and PI staining indicated that knockdown of FAM64A could not impact cell apoptosis. h, i Flow cytometry analysis indicated that knockdown of FAM64A postponed the progression of the cell cycle. j Western blot analysis showed that knockdown of FAM64A downgulated the expression of cycle-related protein cyclin B1 and cyclin D. **P < 0.01, ***P < 0.001.
