Fig. 3: DHT activated the AR to regulate the expression of FAM64A by targeting its promoter sequences.

a The linear correlation analysis was a positive correlation between AR expression and FAM64A expression in prostate cancer patients by TCGA database. b, c The expression of FAM64A was accompanied by increased DHT (10 nM) induced AR at both transcription and translation levels. d–f The mRNA expression of AR and FAM64A in cells transfected with siRNA-AR and DHT (10 nM) at both transcription and translation levels. g Canonical full‐ARE sequences. h The AR‐binding sites in FAM64A promoter from ChIP‐seq results and the prediction by website JASPAR. i, j DNA immunoprecipitated with AR antibody and IgG (as a nonspecific control) were used as templates for PCR using primers that amplified the AR-binding sites within the FAM64A promoter. k The designed mutants of key residues in the ARE4 T-test. l LNCaP and 22Rv1 cells were cotransfected with reporter plasmids (containing the wide type and mutants of ARE), Renilla luciferase reporter gene, and AR expression plasmid and then harvested to measure luciferase activity treat the data as above. *P < 0.05, **P < 0.01, ***P < 0.001.