Fig. 1: Identification of the NSC84167 compound (NSLC01) as synthetically lethal with high NRF2 activation.

A Schematic illustration of the synthetic lethal strategy of targeting cancer cells with high NRF2 activity. B Working flow of identification of NSC84167/NSLC01. C Response curves of seven pancreatic cancer cell lines to NSLC01. Data are presented as mean ± SD, n = 3. D IC50 values of NSLC01 in the seven cell lines. E Western blot analysis of NRF2 and NQO1 expression in pancreatic cancer cell lines. F Correlation of NQO1 expression with the IC50 of NSLC01 in the seven cell lines. G Western blot analysis of the knockdown of NRF2 in PANC1 and ASPC1 cell lines. H Response of PANC1 cells to NSLC01 with NRF2 knockdown (**P < 0.01). I Response of ASPC1 cells to NSLC01 with NRF2 knockdown (*P < 0.05; **P < 0.01). Data in (H) and (I) are mean ± SD, n = 3.