Fig. 1: Hepatocyte-specific Depdc5 deletion induces upregulation of mTORC1 activity and mild liver injury.
From: Depdc5 deficiency exacerbates alcohol-induced hepatic steatosis via suppression of PPARα pathway
Two-month-old LoxP mice and Depdc5-LKO mice were subjected to the following analyses. A, B The strategy for creating Depdc5 hepatocyte-specific knockout mice (A) used in the current study and a representative gel electrophoresis image for genotyping (B). C Western blot and qPCR analyses of DEPDC5 in the livers of control LoxP and LKO mice (n = 4/group). D, E Body weights (D) and liver-weight-to-body-weight ratios (E) of LoxP and LKO mice (n = 5/group). F, G Serum ALT (F) and hepatic TG (G) measurements of LoxP and LKO mice (n = 5/group). H Representative H&E images of liver sections from LoxP and LKO mice; the rectangular boxed areas in upper panels (100×) are magnified as shown in lower panels (400×). I Calculation of the cross-sectional area of zone 3 hepatocytes by ImageJ. J Western blot analysis of liver lysates from overnight fasting or 4 h refeeding LoxP and LKO mice are shown for the indicated phospho-(P) and total proteins. K Quantification of band intensities of phosphorylated proteins normalized to total proteins in (J). Data are presented as mean ± SD. *p < 0.05. In (K), *p < 0.05 vs Fasting LoxP; #p < 0.05 vs Refeeding LoxP. Scale bars: (H) upper, 200 µm; (H) lower, 50 µm.
