Fig. 2: Chronic alcohol consumption reduces DEPDC5 protein while activates mTORC1 signaling in the liver.
From: Depdc5 deficiency exacerbates alcohol-induced hepatic steatosis via suppression of PPARα pathway
Two–three-month-old LoxP and Depdc5-LKO mice were subjected to chronic-plus-binge ethanol feeding (Gao-Binge model) or Lieber-DeCarli liquid diet containing 5% ethanol feeding for 5 weeks (LD 5W model). A, B Western blot (A) and quantification (B) analyses of hepatic mTORC1 signaling, DEPDC5, and KLHL22 in mice treated with Gao-Binge ethanol feeding. C, D Western blot (C) and quantification (D) analyses of hepatic mTORC1 signaling, DEPDC5, and KLHL22 in mice treated with LD 5W ethanol feeding. E, F qPCR analysis of mRNA expression of Depdc5 and Klhl22 genes in the livers of mice treated with Gao-Binge ethanol feeding (E) or LD 5W ethanol feeding (F) and their corresponding pair-fed controls (n = 4–5/group). G, H Western blot and quantification analyses of mTORC1 signaling, DEPDC5, and KLHL22 in the liver samples of normal controls and AH patients. Data are presented as mean ± SD. *p < 0.05.
