Fig. 5: DJ-1 represses p65 nuclear translocation. | Cell Death & Disease

Fig. 5: DJ-1 represses p65 nuclear translocation.

From: DJ-1 inhibits microglial activation and protects dopaminergic neurons in vitro and in vivo through interacting with microglial p65

Fig. 5

A–C BV2 cells were transfected with si-Ctrl or si-DJ-1 for 72 h and then treated with PBS or LPS (100 ng/ml) for 15 min. A The cells were stained with anti-p65 antibody and DAPI. Scale bar, 10 µm. And the percentage of cells with nuclear p65 distribution was quantified. n = 4. B The cells were then subjected to a subcellular fractionation assay. The relative band intensity of cytoplasmic or nuclear p65 to that of GAPDH or H2B was quantified, respectively. n = 3. C Cell lysates were then subjected to immunoblotting. The relative band intensity of IκBα to that of GAPDH was quantified. n = 3. D The supernatants of the BV2 cells transfected with si-Ctrl or si-DJ-1 for 72 h were immunoprecipitated using anti-p65 antibodies or normal rabbit IgG.

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