Fig. 2: TGF-β1-mediated A549-derived exosomes alters the integrity of the BBB in vivo. | Cell Death & Disease

Fig. 2: TGF-β1-mediated A549-derived exosomes alters the integrity of the BBB in vivo.

From: TGF-β1-mediated exosomal lnc-MMP2-2 increases blood–brain barrier permeability via the miRNA-1207-5p/EPB41L5 axis to promote non-small cell lung cancer brain metastasis

Fig. 2

A Exo or Texo is used for tail vein injection per mouse. B, C Rhodamine B and Evan blue were injected into the mouse tail vein 24 h after exosomes injection. After 3 h of circulation, B in vivo cerebral vascular permeability is determined according to the appearance of intravenously injected rhodamine-dextran (red; scale bar, 50 μm). C The absorbance of the Evan blue leakage is detected as OD620 nm/g. D Animals are euthanized, and tissues are harvested 27 h after exosomes injection. Brain tissues are subjected to double-label IF for analysis of CD31 (green) and ZO-1 (red) or CD31 (green) and VE-cadherin (red) expression (scale bar, 50 μm). **p < 0.01.

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