Fig. 3: lnc-MMP2-2 promotes EndoMT, destroys tight junctions, and induces HBMECs monolayer permeability in vitro. | Cell Death & Disease

Fig. 3: lnc-MMP2-2 promotes EndoMT, destroys tight junctions, and induces HBMECs monolayer permeability in vitro.

From: TGF-β1-mediated exosomal lnc-MMP2-2 increases blood–brain barrier permeability via the miRNA-1207-5p/EPB41L5 axis to promote non-small cell lung cancer brain metastasis

Fig. 3

A FISH analysis of lnc-MMP2-2 location in HBMECs. B, C Infection efficiency of lnc-MMP2-2 silencing and overexpression lentivirus in HBMECs detected using qRT-PCR. D, E Expression of EndoMT markers (VE-cadherin, N-cadherin) and of tight junction proteins (ZO-1, occludin, and claudin-5) in lnc-MMP2-2-silenced or -overexpressed HBMECs as measured using IF (scale bar, 50 μm) and western blotting assays. F HBMECs monolayer permeability in stably infected cells with lnc-MMP2-2 knockdown or overexpression. *p < 0.05, **p < 0.01, ***p < 0.001.

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