Fig. 4: lnc-MMP2-2 functions as molecular sponge for miR-1207-5p in HBMECs. | Cell Death & Disease

Fig. 4: lnc-MMP2-2 functions as molecular sponge for miR-1207-5p in HBMECs.

From: TGF-β1-mediated exosomal lnc-MMP2-2 increases blood–brain barrier permeability via the miRNA-1207-5p/EPB41L5 axis to promote non-small cell lung cancer brain metastasis

Fig. 4

A The potential binding sites between miR-1207-5p and lnc-MMP2-2. B, C Stable lnc-MMP2-2-knocked down or -overexpressed HBMEC are subjected to qRT-PCR for analysis of miR-1207-5p expression. D, E lnc-MMP2-2 expression is decreased after transfection with miR-1207-5p inhibitor or agomir. F Complementary sequence between miR-1207-5p and wild-type (Wt) lnc-MMP2-2. The putative binding sites of miR-1207-5p is mutated in lnc-MMP2-2 (Mt lnc-MMP2-2). Luciferase activity is measured in HBMECs that are co-transfected with miR-1207-5p agomir and Wt or Mt lnc-MMP2-2 vector. G Anti-Ago2 RIP is performed in HBMECs transiently overexpressing miR-1207-5p. H The sequences for Wt and Mt forms of miR-1207-5p are shown. lnc-MMP2-2 is highly enriched in the sample pulled down by biotinylated Wt miR-1207-5p but not in Mt miR-1207-5p. **p < 0.01, ***p < 0.001.

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