Fig. 6: TAK-243 disrupts GSCs proliferation and maintenance. | Cell Death & Disease

Fig. 6: TAK-243 disrupts GSCs proliferation and maintenance.

From: RETRACTED ARTICLE: GRP78 determines glioblastoma sensitivity to UBA1 inhibition-induced UPR signaling and cell death

Fig. 6

a Effects of TAK-243 on cell viability in GSC1 and GSC2. b GRP78 and UBA1 expression levels were examined by immunoblotting in GSCs and GBM cells lines. c Cell viabilities were evaluated by CCK-8 assays in GSCs and differentiated GSCs. d Immunoblot analyses of GRP78 levels in GSCs and differentiated GSCs. e−g Effects of TAK-243 on GSCs neurosphere formation, scale bar: 100 μm. h In vitro limiting dilution assays of GSC1 and GSC2 treated with indicated doses of TAK-243 or DMSO. i Representative western blot analysis showing dose-response and time course of the effects of TAK-243 on the polyubiquitylation and ER stress in GSC1 and GSC2, as assessed by immunoblotting for polyubiquitin (polyUb), GRP78, p-PERK(Thr980), PERK, p-eIF2α, eIF2α, ATF4, p-IRE1a(Ser724), IRE1a, CHOP, XBP1s, and ATF6. j The effects of TAK-243 on GSC1 and GSC2 apoptosis as evaluated by Annexin V/PI staining. All the Data are presented as means ± SD. **P < 0.01, ***P < 0.001.

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