Fig. 5: The exosomal miR101 targets CDK8 in mediating IL1A and IL6 expression. | Cell Death & Disease

Fig. 5: The exosomal miR101 targets CDK8 in mediating IL1A and IL6 expression.

From: Hypoxic stress suppresses lung tumor-secreted exosomal miR101 to activate macrophages and induce inflammation

Fig. 5

A The expression of indicated proteins in THP-1 cells co-cultured with control- or CoCl2-treated A549 cells. B THP-1 cells co-cultured with control- or CoCl2-treated A549 cells were transfected with or without miR101 mimic. The expression of CDK8 and SUB1 in THP-1 was analyzed by western blot. C THP-1 cells co-cultured with control- or CoCl2-treated A549 cells were transfected with or without HIF1α siRNA. The expression of CDK8 and SUB1 in THP-1 was analyzed by western blot. D The correlation of CDK8 and SUB1 expression with miR101 expression in the LUSC database. E The survival of patients with lung cancer with different expression levels of CDK8 and SUB1 from Kaplan-Meier plotter (https://kmplot.com/analysis/). F The expression of SUB1 and CDK8 in lung tumors with different levels of HIF1α expression. G The THP-1 cells were transfected with CDK8 siRNA and co-cultured with CoCl2-treated A549 cells. Left, the expression of CDK8 in THP-1 cells; right, the mRNA levels of IL1A and IL6 in THP-1 cells. H The THP-1 cells were transfected with SUB1 siRNA and co-cultured with CoCl2-treated A549 cells. Left, the expression of SUB in THP-1 cells; right, the mRNA levels of IL1A and IL6 in THP-1 cells. # p > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001.

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