Fig. 4: α-synuclein PFFs reduce phagocytic activity in midbrain astrocytes.
From: Fibrillar α-synuclein induces neurotoxic astrocyte activation via RIP kinase signaling and NF-κB
a–e Primary human midbrain astrocyte cultures were treated for 24 h with PFFs or PBS control solution. Cultures were pretreated (30 min) with inhibitors of NF-κB (BAY), JAK/STAT (PYR), or AP1 (SR) signaling prior to the addition of PFFs. a–d Levels of indicated phagocytosis-associated transcripts were measured using qRT-PCR. e Twenty-four hours following PFF or PBS treatment, relative rates of phagocytosis were assessed by measuring uptake of fluorescently labeled zymosan. ns not significant, *p < 0.05, **p < 0.01, ***p < 0.001. Bars represent group means. n = 6 independent replicates for all experiments.
