Fig. 3: Knockdown of Top2 causes defects in eye and head development. | Cell Death & Disease

Fig. 3: Knockdown of Top2 causes defects in eye and head development.

From: Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila

Fig. 3

A–D Knockdown of Top2 in females by three strong RNAi lines using ey-GAL4 causes pupal lethality at 25 °C. A ey/+ control. B–D Phenotypes in the head regions from dead pupae. B ey > Top2 RNAiGD4570. 100% late pupal lethality (N = 52). C ey > Top2 RNAiVSH330177. 100% late pupal lethality (N = 46). (D) ey > Top2 RNAi10223R-2. 94.2% late pupal lethality (N = 52) (All three escapers are females). Scale bars in (A) and (E) are 150 µm. Note that depletion of Top2 in male eye discs by four different RNAi lines (B–D and Top2 RNAiJF01300) causes pupal lethality with similar defects in the head. F–I Variable eye phenotypes of ey > Top2 RNAiJF01300 adult females. E ey/+ control. F no eye (8%, N = 50), (G) Strong reduction (4%), (H) Intermediate reduction (26%), (I) Mild reduction (62%). N number of animals.

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