Fig. 4: Top2 shows genetic interaction with Tctp in organ growth.

A–D Top2^Suo1/+ enhances Tctp RNAi eye phenotype in females. A ey/+ (n = 10, x̅ ± s = 1 ± 0.05), (B) Top2^Suo1/+ (n = 10, x̅ ± s = 0.933 ± 0.034), (C) ey > Tctp RNAi/+ (n = 10, x̅ ± s = 0.687 ± 0.06), (D) ey > Tctp RNAi/Top2^Suo1 (n = 8, x̅ ± s = 0.569 ± 0.069). Scale bar in (A) is150 µm. E Quantification of relative eye sizes in (A–D). Statistical analysis in E by unpaired two-tailed student t-test, **P < 0.01 and ****P < 0.0001. Error bars in (E) are SD. F–M Wing phenotypes of Tctp RNAi and Top2^Suo1/+ in males (F–I) and females (J–M). F, J nub/+ control, (G, K) Top2^Suo1/+, H, L Intermediate phenotype showing wing size reduction, (I, M) Strong phenotype showing size reduction with severe folding. Scale bars in (F), (J) are 300 µm. N, O Genetic interaction between Tctp RNAi and Top2^Suo1/+ in males (N) and females (O). Top2^Suo1/+ weakly enhances the intermediate phenotype to the strong phenotype in males (N) while strongly enhancing it in females (O). N number of animals.