Fig. 5: Tctp RNAi reduces Top2 protein levels. | Cell Death & Disease

Fig. 5: Tctp RNAi reduces Top2 protein levels.

From: Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila

Fig. 5

A–A’’’ en > GFP/+ shows no significant change in the Top2 level in the posterior domain at 25 °C. A GFP, (A’) DAPI, (A’’) Top2, (A’’’) Merge. Scale bars in (A–D) are 50 µm. B-B’’’ en > GFP > Tctp i shows a decrease in Top2 level at 25 °C. (11/14 discs; 78.6%) (B) GFP, (B’) DAPI, (B’’) Top2, (B’’’) Merge. The white arrow shows an area of decreased Top2 in the posterior region. C–C’’’ en > GFP > Diap1 shows little change in the Top2 level at 25 °C. (C) GFP, (C’) DAPI, (C’’) Top2, (C’’’) Merge. D–D’’’ en > GFP > Tctp i > Diap1 shows rescue of Top2 level at 25 °C (8/12 discs; 75%). D GFP, (D’) DAPI, (D’’) Top2, (D’’’) Merge. E, F Western blots stained for Top2, Tctp, and β-Tubulin. (E) Top2 protein level is decreased in Tctp-depleted S2 cells (representative of three independent experiments). F Tctp protein level is decreased in Top2-depleted S2 cells (representative of three independent experiments).

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