Fig. 6: Influence of hsa_circ_0003222 and miR-527 in vivo. | Cell Death & Disease

Fig. 6: Influence of hsa_circ_0003222 and miR-527 in vivo.

From: hsa_circ_0003222 accelerates stemness and progression of non-small cell lung cancer by sponging miR-527

Fig. 6

A Representative images of xenograft tumors isolated from nude mice in the different groups. B Tumor sizes in different groups. C HE staining in xenograft tumors tissue. D Immunohistochemistry of Ki-67 in xenograft tumors tissue. E TUNEL assay in xenograft tumors tissue. F–H RT-qPCR demonstrating hsa_circ_0003222, miR-527, and PHF21B levels in xenograft tumors tissue. I Western blot demonstrating CD44, CD133, OCT4, SOX2, PD-L1, PHF21B, and β-catenin levels in xenograft tumors tissue. J Immunofluorescence showing the expression of CD44, CD133, OCT4, and SOX2 in xenograft tumors tissue. K Representative images of orthotopic model tumors isolated from nude mice in the different groups. L Representative HE staining of orthotopic model tumors isolated from nude mice in the different groups. M Live imaging demonstrating hsa_circ_0003222 and miR-527 effects on the metastasis of PC9 LCSCs 30 days after intravenous tail injection. N Representative images of xenograft tumors isolated from nude mice in the different groups after treatment with anti-PD-L1. O Tumor sizes in the various groups. Data were presented as mean ± SD; *P < 0.05, **P < 0.01.

Back to article page